BioSciences Division Argonne National Laboratory Argonne, IL 60439 phone: (630) 252-3280 fax: (630) 252-9793 e-mail knemec@anl.gov

EDUCATION:                B.A. Psychology, A.L.P.A. Business, University of Notre Dame, 1988
                                    M.S. Teaching of Chemistry, University of Illinois in Champaign-Urbana, 1992
                                    Ph.D. Physiology and Biophysics, University of Alabama at Birmingham, 1997

EXPERIENCE

      ARGONNE NATIONAL LABORATORY                                                                                                2004-Present

      Energy Systems Division (2004-2005), Biosciences Division (2005-present)

      Title:  Visisting Scientist / Microbiologist

Responsibilities include: 1) examining the susceptibility of varying materials to biodegradation; 2) growing and maintaining biofilms for the development of novel imaging and mass spectrometry techniques; 3) purifying glucose fructose oxidoreductase for a separative bioreactor the lab created; 4) measuring iron reduction and U^VI reduction by bacteria; and 5) characterizing exopolysaccharides and other exuded compounds by soil bacteria.

NATIONAL CENTER FOR AGRICULUTURAL UTILIZATION RESEARCH                                                  2002-2004

Fermentation Biotechnology Division

Title: Research Microbiologist

Responsibilities included: 1) acquiring field samples from fuel ethanol plants, 2) isolating, identifying, maintaining and storing contaminating bacterial species found in the field samples, 3) characterization of biofilms produced by the bacteria isolated, and 4) studying the antimicrobial susceptibility of the isolated bacterial species.

                       Richwoods high school                                                                                                                2001-2002

Science Department   

Title: Teacher

Responsibilities included: 1) teaching Advanced Placement Chemistry, Honors Chemistry and Physics; and 2) writing part of the curriculum for the International Baccalaureate chemistry program for the school.

wake forest university school of medicine                                                                         1997-2000

Atherosclerosis Department

Title:  Post-doctoral Fellow

Responsibilities included: 1) Studying N-deactylase/N-sulfotransferase enzyme differences and integrin expression variation between show racer and white carneau pigeon smooth muscle cells; 2) Tested ACAT inhibitors, in vitro and on whole cells; 3) Selected to develop a high throughput method to aid in study of enzymes involved in cholesterol ester pathways; and 4) Trained graduate students on assays and tissue culture techniques.

u. of alabama at birmingham                                                                                                      1992-1997

Anesthesiology Department

Title: Research Assistant 1992-1993   Doctoral Student 1993-1997    

Responsibilities included: 1) Studing interactions of superoxide and nitric oxide free radicals following liver ischemia­­/reperfusion; 2) Developed and demonstrated effects of a novel nitrosation product of uric acid on vaso­relaxation properties of rat thoracic aorta; 3) co-wrote patent on antibodies against aldehyde oxidase; 4) Developed an HPLC method for nitrotyrosine; and 5) coordinated liver transplants, acquired donor and recipient samples.

u. of illinois at champaign-urbana                                                                                           1988-1992

Chemistry Department

Title: Teaching Assistant

Responsibilities included: 1) Developing all lesson plans, lectures, quizzes, test administration, grading, and laboratory instruction; and 2) a master’s thesis on the effects of four different learning environments.

PUBLICATIONS/PRESENTATIONS:

Published Manuscripts:                                                                                                                           21 (11 first author)

Presentations delivered to scientific audiences:                                                                                         30 (7 by specific invitation)

SELECTED PUBLICATIONS:

        Edirisinghe PD, Moore JF, Skinner-Nemec KA, Lindberg C, Giometti CS, Veryovkin IV, Hunt J, Pellin MJ, and Hanley L.  Detection of in-situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionization mass spectrometry, submitted to Analytical Chemistry, 2006.

        Skinner KA, Tan S, and Parks DA. Uric acid metabolism, version 2.0 (A3910). In: Embryonic Encyclopedia of Life Sciences. http://www.els.net, by Nature/Scientific American, 2005.

       Skinner KA and Leathers TD.  Comparison of identification methods for Lactobacillus, Proc. Corn Util. Technol. Conf. 1 p. 2004.

        Skinner KA and Leathers TD.  Biofilm formation by bacterial contaminants of fuel ethanol production.  Proc. Biofilms 2004: Structure and Activity of Biofilms p. 210-214, 2004.

        Skinner KA and Leathers TD.  Bacterial contaminants of fuel ethanol production.  J. Indus. Microbiol. Biotechnol. 31:401-408, 2004.

        Skinner KA, Khoo KH and Parks DA.  Uric acid metabolism.  In: Encylopedia of Life Sciences.  2nd Edition.  Edited by Robertson S. Nature Publishing Group, New York, 2004.

        Skinner KA, Tan S, and Parks DA. Uric acid metabolism. In: Encyclopedia of Life Sciences. Edited by Robertson S. Macmillan Reference Limited, Stockton Press, London, 2001, 1-8.

        Lee RG, Willingham M, Davis M, Skinner KA, and Rudel LL. Differential expression of ACAT1 and ACAT2 among cells within liver, intestine, kidney and adrenal of nonhuman primates. J. Lipid Res. 41(12) 1991-2001,2000.

        Joyce CW, Shelness GS, Davis MA, Lee RG, Skinner K, Anderson RA, and Rudel LL. ACAT1 and ACAT2 membrane topology segregates a serine essential for activity to opposite sides of the endoplasmic reticulum membrane. Molec. Bio. Cell, 11(11):3675-3687, 2000.

        Skinner KA, Tan S, and Parks DA. Uric acid metabolism. In: Embryonic Encyclopedia of Life Sciences. http://www.els.net, by Nature/Scientific American, 1999.

        Parks DA, Skinner KA, Gelman S, and Maze M. Hepatic Physiology. In: Anesthesia, 5th Edition. Edited by Miller RD, Churchill Livingstone, Philadelphia, 1:647-661, 1999.

        Parks DA, Skinner KA, Tan S, and Skinner HB. Xanthine oxidase in biology and medicine. In Reactive Oxygen Species in Biological Systems: Selected Topics, Gilbert DL, Colton CA, eds., Kluwer Academic/Plenum Publishers, New York, 1999, pp. 397-420.

        Joyce C, Skinner K, Anderson RA, and Rudel LL. Acyl-coenzyme A:cholesterol acyltransferase 2. Current Opinion in Lipidology 1999, 10(2):89-95.

        Skinner KA, White CR, Rakesh P, Tan S, Barnes S, Kirk M, Darley-Usmar V, Parks DA. Nitrosation of uric acid by peroxynitrite: formation of a vasoactive nitric oxide donor. J. Biol. Chem., 273(38): 24491-24498, 1998.

        Parks DA, Skinner KA, Skinner HB and Tan S. Multiple organ dysfunction syndrome: role of xanthine oxidase and nitric oxide. Pathophysiology, 5(1):49-56, 1998.

        Hassoun PM, Yu FS, Cote CG, Zulueta JJ, Sawhney R, Skinner KA, Skinner HB, Parks DA, and Lanzillo JJ. Upregulation of xanthine oxidase by LPS, interleukin-1, and hypoxia: role in acute lung injury. Am. J. Respir. Crit. Care Med., 158:299-305,1998.

        Skinner KA, Crow JP, Skinner HB, Chandler RT, Thompson JA, Parks DA. Free and protein associated nitrotyrosine formation following rat liver preservation and transplantation. Arch. Biochem. Biophys., 342:282-288, 1997.

        Tan S, Liu, Y-Y, Nielsen, VG, Skinner K, Kirk KA, Baldwin ST, Parks DA. Maternal infusion of antioxidants (Trolox and ascorbic acid) protects the fetal heart in rabbit fetal hypoxia. Pediatric Research 39:499-503, 1996.

        Weinbroum A, Nielsen VG, Tan S, Gelman S, Matalon S, Skinner KA, Bradley E Jr, Parks DA. Liver ischemia-reperfusion increases pulmonary permeability in rat: role of circulating xanthine oxidase. Am. J. Physiol. (Gastrointestinal and Liver Physiology) 268:G988-G996, 1995.

PROFESSIONAL SOCIETIES

The Oxygen Society, American Physiologic Society, American Chemical Society, International Water Association

HONORS/AWARDS

Co-Exhibition Chair for American Chemical Society meeting held in Peoria in 2004

American Heart Association Postdoctoral Fellowship, 2000-2002

Oxygen Society, Young Investigator Award, 1997

Outstanding Teaching Assistant Award, 1990

Assigned as Head Teaching Assistant, 1989

Graduate and Postdoctoral Advisors and Advisees:  DA Parks (U. AL at B’ham) and TD Leathers (NCAUR/USDA)